View online or download applied biosystems 7500 installation and maintenance manual, getting started, getting started manual. About the 7300plus instrument the applied biosystems 7300plus realtime pcr instrument is a 96well, fourcolor. The 7300 and 7500 realtime pcr systems taqman rnase p instrument verification plate satisfies the same requirements as the applied biosystems 7300 and 7500 realtime pcr systems. Applied biosystems 7300 installation and maintenance manual. Applied biosystems 7500 fast, 7500 and 7300 realtime pcr systems. Applied biosystems qpcr analysis modules within the thermo fisher cloud are web browserbased software tools that are flexible, fast and easy to use. Applied biosystems 7500 fast and 7500 realtime pcr. The hrm software is available on the 7500 fast real time pcr system. Used applied biosystems 7300 realtime pcr system 600.
Applied biosystems 7500 fast realtime pcr systems spectral calibration kit ii easytouse, preloaded, optical reaction plates enable quick and easy calibration of the 7500 fast realtime pcr system. Applied biosystems 7300 7500 real time pcr system allelic discrimination getting started guide pn 4347822 applied biosystems 7300 7500 real time pcr system. Relative quantitation of gene expression pn 4303859 send us your comments. We have 3 applied biosystems 7300 manuals available for free pdf download. Data using realtime quantitative pcr and the 2ct method. The great thing about the abi 7300 real time pcr is theres no need to purify pcr, run any gels, or implement post pcr. Audience this guide is intended for novice and experienced applied biosystems 7300, 7500, and. Applied biosystems 7300 75007500 fast real time pcr system installation and maintenance guide 4347828 4378657 real time pcr systems chemistry guide 4348358 4378658 applied biosystems 7500 fast real time pcr system, qrc 4362285 4378659 applied biosystems real time system computer set up guide, qrc 4365367 4378660. The acceptable volume range is 20100l which saves time when compare to manual pcr. Prior to downloading any software to a computer used for instrument controloperation, please consult with your life technologies service. New york sarscov2 realtime reverse transcriptase rt. Applied biosystems stepone and steponeplus realtime pcr systems reagent guide vii preface how to use this guide about the system documentation the guides listed below are shipped with the applied biosystems stepone and steponeplus realtime pcr systems stepone and steponeplus systems. New york sarscov2 realtime reverse transcriptase rt pcr.
Applied biosystems 7300 real time pcr system the abi 7300 is a reliable tool which combines thermal cycling with fluorescence detection that measures cycle by cycle accrual. Applied biosystems quantstudio 6 flex realtime pcr system, 96well fast, laptop facilitates realtime pcr based applications with an upgradable block selection, allowing you to plan for experiments today and grow with tomorrows needs. The software information in this document is applicable to realtime pcr software v2. The fourcolor detection system combines thermal cycling with fluorescence detection that measures cycle by cycle accrual. Its specialized optical system enables easy and accurate calibration to new dyes without requiring addition of new filer sets. Applied biosystems 7300 realtime pcr system thermo. The applied biosystems 7500 realtime pcr system is a 96well, fivecolor platform that uses fluorescencebased polymerase chain reaction pcr reagents to analyze items. The applied biosystems 7500 fast real time pcr system allows the user to perform highspeed thermal cycling giving run times for quantitative real time pcr applications such as relative quantitation in fewer than 40 minutes. This manual is written for principal investigators and laboratory staff who. Detection system user guide, the abi prism 7700 sequence detection system users manual, the geneamp 5700 sequence detection system users manual, the applied biosystems 73007500 re al time pcr system relative quantitation getting started guide, the applied biosystems 73007500 real time pcr system.
The 7300 and 7500 real time pcr systems taqman rnase p instrument verification plate is a 96well pcr plate preloaded with the necessary reagents to detect and quantitate genomic copies of the. Complete realtime pcr runs in less than two hours with the abi 7300 real time using tubes, 96 well plates, 8strip tubes. Through an advanced multicolour detection alogorithm the system can analyse up to 4 dyes. Complete realtime pcr runs with the abi 7300 real time using tubes, 96 well plates, 8strip tubes.
Applied biosystems 7300 sequence detection system real. Applied biosystems 7300 7500 real time pcr system online help applied biosystems 7300 7500 real time pcr system allelic discrimination getting started guide pn 4347822 applied biosystems 7300 7500 real time pcr system plusminus getting started guide pn 4347821. This manual is written for principal investigators and laboratory staff. In 20012010, rtpcr has been used as a powerful tool for genotyping applied biosystems abi7900 fast realtime pcr system user guide, biosystems 730075007500, alker, 2004, quantifying viral load, and gene copy number assays. The calibrate function is also available in the control console. For the applied biosystems 7500 real time pcr system and the stratagene mx3000p, the use of rox reference dye lmp is recommended. The abi 7300 is great quantifying nucleic acid sequences without compromising data. Specifically designed with a user friendly, yet powerful interface, they are ideal for both new and experienced real time pcr.
Applied biosystems 7300 real time pcr system youtube. Applied biosystems 7500 fast and 7500 realtime pcr system. We also offer the 7500 fast dx instrument for your diagnostic needs. The technical manual listed below, complete protocol, describes the plexor hy system and provides instructions for use with the applied biosystems 7500 and 7500 fast realtime pcr systems, including reaction setup, realtime pcr instrument programming and data analysis using the plexor analysis software. This system is a sophisticated platform for users who require extended capabilities and maximum versatility. This manual is written for principal investigators and laboratory staff who conduct absolute quantification assays using the applied biosystems 730075007500. About the 75007500 fast system the applied biosystems 75007500 fast real time pcr system uses fluorescencebased polymerase chain reaction pcr reagents to provide. Applied biosystems 7300 realtime pcr system thermo fisher. Results you can trustevery time the applied biosystems 7300 system offers both proven reliability and superior data qualityexactly what you would expect from the leader in realtime pcr systems. The applied biosystems 7300 real time pcr runs on its own software platform and quantitative results are available immediately, once pcr run is complete.
Prior to downloading any software to a computer used for instrument controloperation, please consult with your life technologies service and support representative. The 7500 real time pcr system is a powerful platform for labs requiring superior performance and maximum dye versatility. Through an advanced multicolour detection alogorithm the system. Applied biosystems 7500 realtime pcr systems offer you high performance, multicolor realtime pcr wherever your research takes you. The software provides projectbased analysis of real time and endpoint data for various quantitative and qualitative pcr. Applied biosystem 7300 real time pcr system andbio. The applied biosystems 7300, 7500 and 7500 fast realtime pcr systems are realtime thermal cyclers covered by us patents and corresponding claims in their nonus counterparts, owned by applied biosystems. The applied biosystems 7300 real time pcr system is a real time thermal cycler covered by us patents and claims in nonus counterparts. Applied biosystems abi 7300 pcr system general for sale. Applied biosystems 73007500 real time pcr system getting started guide 2. Applied biosystem 7300 real time pcr system is a reliable tool which combines thermal cycling with fluorescence detection that measures cycle by cycle accrual.
This instrument is capable of quantitative detection using real time. Applied biosystems 7300 real time pcr system andbio. Applied biosystems absolute quantitation manual reverse. For the applied biosystems 7500 real time pcr system. Refurbished applied biosystems 7300 real time pcr system w. The system uses standard 96well format and filter wheel with four emission filter which allow the detection of all dyes between 510 and 650nm. Ground the instrument according to the provided instructions.
Applied biosystems abi 7500 real time pcr video youtube. Applied biosystems 730075007500 fast real time pcr system site preparation guide v preface how to use this guide purpose of this guide the applied biosystems 730075007500 fast real time pcr system site preparation guide provides the information you need to fully prepare your site for the arrival and installation of the applied biosystems 730075007500 fast real. Applied biosystems 75007500 fast thermo fisher scientific. The great thing about the abi 7300 realtime pcr is theres no need to purify pcr, run any gels, or implement post pcr alterations. The applied biosystems 7300 real time pcr is an affordable solution for labs with a small budget.
The applied biosystems high resolution melting hrm software is the easiest to use melting analysis software enabling realtime pcr melt curve assays to be used more accurately for mutation scanning and genotyping. Applied biosystems taqman rnase p instrument verification. This system is a sophisticated platform for users who require extended. Pcr and thermal cyclers from applied biosystems listing. Quantitative detection of target nucleic acid sequences targets using real time. Rtpcr has been the gold standard of gene expression level assay.
Applied biosystems 7500 real time pcr systems offer you high performance, multicolor real time pcr wherever your research takes you. Applied biosystems 7300 7500 real time pcr system getting started guide 2. Applied biosystems 730075007500 fast system setup and maintenance video cd provided with the instrument. The applied biosystems 7300 realtime pcr system is a realtime thermal cycler covered by us patents and claims in nonus counterparts. The applied biosystems 7300 realtime pcr system is a real time.
Applied biosystems 73007500 real time pcr system online help applied biosystems 73007500 real time pcr system allelic discrimination getting started guide pn 4347822 applied biosystems 73007500 real time pcr system plusminus getting started guide pn 4347821. The use of rox reference dye lsr or lmp is optional. Real value the applied biosystems 7300 real time pcr system log of change in normalized reporter. Plexor hy system for the applied biosystems 7500 and 7500. The applied biosystems 7500 is todays top choice in rt pcr. Applied biosystems realtime pcr systems, including. The applied biosystems 75007500 fast realtime pcr system guide provides the information you need to maintain your applied biosystems 75007500 fast realtime pcr system. Specifically designed with a user friendly, yet powerful interface, they are ideal for both new and experienced realtime pcr researchers. The 7300 is a real time pcr system with fourcolor detection capability. Applied biosystems 7500 fast realtime pcr systems spectral. Applied biosystems quantstudio 5 realtime pcr system, 96.
Applied biosystems 7500 fast, 7500 and 7300 realtime pcr. Pcr instrument applied biosystems 7300plus realtime. Applied biosystems 730075007500 fast realtime pcr system installation and. No right is conveyed expressly, by implication or by estoppel under any other patent claim, such as claims to apparatus, reagents, kits, or methods such as 5 nuclease methods. Aug 19, 2016 applied biosystems 7300 real time pcr system available for sale at the lab world group. Applied biosystems 7300 75007500 fast real time pcr system installation and maintenance guide xi. Real value the applied biosystems 7300 realtime pcr system log of change in normalized reporter. Page 1 applied biosystems 7300 75007500 fast real time pcr system before starting installation and the installation maintenance guide unpacking and setting up the instrument installing the software and powering on the instrument performing the regions of interest roi calibration performing the background calibration and. Abi prism 7000 sequence detection systems relative.
For more information about using the 73007500 system, refer to. Applied biosystems quantstudio 6 flex realtime pcr system. This manual is written for principal investigators and laboratory staff who conduct. The applied biosystems analysis software is a secure web application for analysis of data that are generated on thermo fisher scientific real time pcr instruments. Applied biosystems 7300 real time pcr system is the perfect entry to real time pcr, producing highend results. Built on over 10 years of realtime expertise, the applied biosystems 7300 realtime pcr system is an affordable platform for the detection and quantification of nucleic acid sequences that will not compromise your data quality or dye choice flexibility. Applied biosystems 73007500 realtime pcr system plusminus getting started. Applied biosystems 730075007500 fast realtime pcr system. Biosystems 7300 7500 real time pcr system absolute quantitation getting started guide, or the applied biosystems 7300 7500 real time pcr system installation and maintenance getting started. Applied biosystems 7300 75007500 fast real time pcr system installation and maintenance guide part number 4347828 rev. Used applied biosystems 7300 real time pcr 2030573. Applied biosystems 7300 75007500 fast real time pcr system absolute quantification getting started guide ix how to obtain more information related documentation for more information about using the 7300 75007500 fast system, refer to the applied biosystems 7300 75007500 fast real time pcr system online help or the documents shown below. Feb 24, 2015 the abi 7500 is a real time pcr that delivers accurate results in less than 30 minutes. Applied biosystems 75007500 fast realtime pcr system site preparation guide applied biosystems 75007500 fast realtime.
Results you can trustevery time the applied biosystems 7300 system offers both proven reliability and superior data qualityexactly what you would expect from the leader in real time pcr systems. Northern blotting or rnase protection assays, realtime pcr offers enormous timesavings, greater. View and download applied biosystems 7300 installation and maintenance manual online. Applied biosystems 7300 sequence detection system real time pcr system in london, united kingdom.
For more information about using the 7300 7500 system, refer to. Applied biosystems 7300 manuals manuals and user guides for applied biosystems 7300. Description the applied biosystems 7300 7500 real time pcr system 7300 7500 system uses fluorescentbased pcr chemistries to provide quantitative detection of nucleic acid sequences using realtime analysis. View online or download applied biosystems 7300 installation and maintenance manual, getting started manual. Applied biosystems 75007500 fast real time pcr system getting started guide for genotyping experiments v preface how to use this guide about the system documentation the guides listed below are shipped with the applied biosystems 75007500 fast real time pcr system 75007500 fast system. The abi 7500 is a real time pcr that delivers accurate results in less than 30 minutes. Applied biosystems 7500 realtime pcr system, desktop. The applied biosystems 7300, 7500 and 7500 fast realtime pcr systems.
E for instructions and further guid ance on pure dye calibration. Applied biosystems 730075007500 fast realtime pcr system and steponeplus realtime pcr system. Applied biosystems 7300 real time pcr system available for sale at the lab world group. Getting started guide relative quantification applied biosystems 7300 75007500 fast real time pcr system introduction and example rq experiment. To achieve high quality results of qpcr, a superb apparatus is needed and this is where you can turn to the applied biosystems 7300 real time pcr system. It is suitable for a wide range of applications, including gene expression analysis, snp genotyping, and pathogen quantitation. The hrm software is available on the 7500 fast realtime pcr system, which delivers precise results in a standard 96well format. Detection kit, the applied biosystems 7500 fast dx real time pcr instrument must be calibrated by the manufacturer to read the following dyes. It the 7300 is a realtime pcr system with fourcolor detection capability.
The applied biosystems high resolution melting hrm software is the easiest to use melting analysis software enabling real time pcr melt curve assays to be used more accurately for mutation scanning and genotyping. The 7300 is a realtime pcr system with fourcolor detection capability. Abi prsi m 700077007900ht sequence detection systems and the applied biosystems 7300 real time pcr system, the use of rox reference dye lsr 50x is recommended. Applied biosystems 75007500 fast realtime pcr system getting.
Installation and maintenance guide pn 4347828e thermo fisher. This small but robust pcr takes up less space than the applied biosystems 7900ht. The applied biosystems family of realtime solutions combines innovative thermal cycling systems, powerful software, optimized reagents, your choice of. In addition, the abi 7300 works with a number of different dye choices. Relative quantification getting started guide for the applied.
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